Abstract: The properties of the Lambda(1405) resonance are key ingredients for determining the antikaon-nucleon interaction in strangeness nuclear physics, and the novel internal structure of the Lambda(1405) is of great interest in hadron physics, as a prototype case of a baryon that does not fit into the simple three-quark picture. We show that a quantitative description of the antikaon-nucleon interaction with the Lambda(1405) is achieved in the framework of chiral SU(3) dynamics, with the help of recent experimental progress. Further constraints on the (K) over barN subthreshold interaction are provided by analyzing pi Sigma spectra in various processes, such as the K(-)d -> pi Sigma n reaction and the Lambda(c) -> pi pi Sigma decay. The structure of the Lambda(1405) is found to be dominated by an antikaon-nucleon molecular configuration, based on its wavefunction derived from a realistic (K) over barN potential and the compositeness criteria from a model-independent weak-binding relation.