Abstract: The Inverse Seesaw naturally explains the smallness of neutrino masses via an approximate B-L symmetry broken only by a correspondingly small parameter. In this work the possible dynamical generation of the Inverse Seesaw neutrino mass mechanism from the spontaneous breaking of a gauged U(1) B-L symmetry is investigated. Interestingly, the Inverse Seesaw pattern requires a chiral content such that anomaly cancellation predicts the existence of extra fermions belonging to a dark sector with large, non-trivial, charges under the U(1) B-L. We investigate the phenomenology associated to these new states and find that one of them is a viable dark matter candidate with mass around the TeV scale, whose interaction with the Standard Model is mediated by the Z' boson associated to the gauged U(1) B-L symmetry. Given the large charges required for anomaly cancellation in the dark sector, the B-L Z' interacts preferentially with this dark sector rather than with the Standard Model. This suppresses the rate at direct detection searches and thus alleviates the constraints on Z'-mediated dark matter relic abundance. The collider phenomenology of this elusive Z' is also discussed.