Di Gregorio, E., Staelens, M., Hosseinkhah, N., Karimpoor, M., Liburd, J., Lim, L., et al. (2024). Raman Spectroscopy Reveals Photobiomodulation-Induced α-Helix to β-Sheet Transition in Tubulins: Potential Implications for Alzheimer's and Other Neurodegenerative Diseases. Nanomaterials, 14(13), 1093–21pp.
Abstract: In small clinical studies, the application of transcranial photobiomodulation (PBM), which typically delivers low-intensity near-infrared (NIR) to treat the brain, has led to some remarkable results in the treatment of dementia and several neurodegenerative diseases. However, despite the extensive literature detailing the mechanisms of action underlying PBM outcomes, the specific mechanisms affecting neurodegenerative diseases are not entirely clear. While large clinical trials are warranted to validate these findings, evidence of the mechanisms can explain and thus provide credible support for PBM as a potential treatment for these diseases. Tubulin and its polymerized state of microtubules have been known to play important roles in the pathology of Alzheimer's and other neurodegenerative diseases. Thus, we investigated the effects of PBM on these cellular structures in the quest for insights into the underlying therapeutic mechanisms. In this study, we employed a Raman spectroscopic analysis of the amide I band of polymerized samples of tubulin exposed to pulsed low-intensity NIR radiation (810 nm, 10 Hz, 22.5 J/cm2 dose). Peaks in the Raman fingerprint region (300-1900 cm-1)-in particular, in the amide I band (1600-1700 cm-1)-were used to quantify the percentage of protein secondary structures. Under this band, hidden signals of C=O stretching, belonging to different structures, are superimposed, producing a complex signal as a result. An accurate decomposition of the amide I band is therefore required for the reliable analysis of the conformation of proteins, which we achieved through a straightforward method employing a Voigt profile. This approach was validated through secondary structure analyses of unexposed control samples, for which comparisons with other values available in the literature could be conducted. Subsequently, using this validated method, we present novel findings of statistically significant alterations in the secondary structures of polymerized NIR-exposed tubulin, characterized by a notable decrease in alpha-helix content and a concurrent increase in beta-sheets compared to the control samples. This PBM-induced alpha-helix to beta-sheet transition connects to reduced microtubule stability and the introduction of dynamism to allow for the remodeling and, consequently, refreshing of microtubule structures. This newly discovered mechanism could have implications for reducing the risks associated with brain aging, including neurodegenerative diseases like Alzheimer's disease, through the introduction of an intervention following this transition.
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Kalliokoski, M., Mitsou, V. A., de Montigny, M., Mukhopadhyay, A., Ouimet, P. P. A., Pinfold, J., et al. (2024). Searching for minicharged particles at the energy frontier with the MoEDAL-MAPP experiment at the LHC. J. High Energy Phys., 04(4), 137–22pp.
Abstract: The MoEDAL's Apparatus for Penetrating Particles (MAPP) Experiment is designed to expand the search for new physics at the LHC, significantly extending the physics program of the baseline MoEDAL Experiment. The Phase-1 MAPP detector (MAPP-1) is currently undergoing installation at the LHC's UA83 gallery adjacent to the LHCb/MoEDAL region at Interaction Point 8 and will begin data-taking in early 2024. The focus of the MAPP experiment is on the quest for new feebly interacting particles – avatars of new physics with extremely small Standard Model couplings, such as minicharged particles (mCPs). In this study, we present the results of a comprehensive analysis of MAPP-1's sensitivity to mCPs arising in the canonical model involving the kinetic mixing of a massless dark U(1) gauge field with the Standard Model hypercharge gauge field. We focus on several dominant production mechanisms of mCPs at the LHC across the mass-mixing parameter space of interest to MAPP: Drell-Yan pair production, direct decays of heavy quarkonia and light vector mesons, and single Dalitz decays of pseudoscalar mesons. The 95% confidence level background-free sensitivity of MAPP-1 for mCPs produced at the LHC's Run 3 and the HL-LHC through these mechanisms, along with projected constraints on the minicharged strongly interacting dark matter window, are reported. Our results indicate that MAPP-1 exhibits sensitivity to sizable regions of unconstrained parameter space and can probe effective charges as low as 8 x 10 -4 e and 6 x 10 -4 e for Run 3 and the HL-LHC, respectively.
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MoEDAL Collaboration(Acharya, B. et al), Mitsou, V. A., Musumeci, E., Papavassiliou, J., Ruiz de Austri, R., Staelens, M., et al. (2024). MoEDAL Search in the CMS Beam Pipe for Magnetic Monopoles Produced via the Schwinger Effect. Phys. Rev. Lett., 133(7), 071803–7pp.
Abstract: We report on a search for magnetic monopoles (MMs) produced in ultraperipheral Pb-Pb collisions during Run 1 of the LHC. The beam pipe surrounding the interaction region of the CMS experiment was exposed to 184.07 μb-1 – 1 of Pb-Pb collisions at 2.76 TeV center-of-mass energy per collision in December 2011, before being removed in 2013. It was scanned by the MoEDAL experiment using a SQUID magnetometer to search for trapped MMs. No MM signal was observed. The two distinctive features of this search are the use of a trapping volume very close to the collision point and ultrahigh magnetic fields generated during the heavy-ion run that could produce MMs via the Schwinger effect. These two advantages allowed setting the first reliable, world-leading mass limits on MMs with high magnetic charge. In particular, the established limits are the strongest available in the range between 2 and 45 Dirac units, excluding MMs with masses of up to 80 GeV at a 95% confidence level.
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Kalliokoski, M., Levi, G., Maulik, A., Ostrovskiy, I., Patrizii, L., Pinfold, J., et al. (2025). Calibration of Solid State Nuclear Track Detectors for rare event searches. J. Instrum., 20(3), P03014–12pp.
Abstract: The calibration of the CR39 (R) and Makrofol (R) Nuclear Track Detectors of the MoEDAL experiment at the CERN-LHC was performed by exposing stacks of detector foils to heavy ion beams with energies ranging from 340 MeV/nucleon to 150 GeV/nucleon. After chemical etching, the base areas and lengths of etch-pit cones were measured using automatic and manual optical microscopes. The response of the detectors as measured by the ratio of the track-etching rate over the bulk-etching rate, was determined over a range extending from their threshold at Z/beta 7 and 50 for CR39 and Makrofol, respectively, up to Z/beta 92.
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MoEDAL Collaboration(Acharya, B. et al), Mitsou, V. A., Musumeci, E., Papavassiliou, J., Ruiz de Austri, R., Staelens, M., et al. (2025). Search for Highly Ionizing Particles in pp Collisions during LHC Run 2 Using the Full MoEDAL Detector. Phys. Rev. Lett., 134(7), 071802–8pp.
Abstract: This search for magnetic monopoles (MMs) and high electric charge objects (HECOs) with spins 0, 1/2, and 1, uses for the first time the full MoEDAL detector, exposed to 6.46 fb(-1) proton-proton collisions at 13 TeV. The results are interpreted in terms of Drell-Yan and photon-fusion pair production. Mass limits on direct production of MMs of up to 10 Dirac magnetic charges and HECOs with electric charge in the range 10e to 400e, were achieved. The charge limits placed on MM and HECO production are currently the strongest in the world. MoEDAL is the only LHC experiment capable of being directly calibrated for highly ionizing particles using heavy ions and with a detector system dedicated to definitively measuring magnetic charge.
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