Abstract: We present the first lattice study of pion-pion scattering with varying number of colors, N-c. We use lattice simulations with four degenerate quark flavors, N-f = 4, and N-c= 3 – 6. We focus on two scattering channels that do not involve vacuum diagrams. These correspond to two irreducible representations of the SU(4) flavor group: the fully symmetric one, SS, and the fully antisymmetric one, AA. The former is a repulsive channel equivalent to the isospin-2 channel of SU(2). By contrast, the latter is attractive and only exists for N-f >= 4. A representative state is (vertical bar D-s(+) pi(+)> – vertical bar D+ K+ >) /root 2. Using Lfischer's formalism, we extract the near-threshold scattering amplitude and we match our results to Chiral Perturbation Theory (ChPT) at large N-c. For this, we compute the analytical U(N-f) ChPT prediction for two-pion scattering, and use the lattice results to constrain the N-c scaling of the relevant low-energy couplings.