Abstract: Empirical knowledge of the fitness effects of mutations is important for understanding many evolutionary processes, yet this knowledge is often hampered by several sources of measurement error and bias. Most of these problems can be solved using site-directed mutagenesis to engineer single mutations, an approach particularly suited for viruses due to their small genomes. Here, we used this technique to measure the fitness effect of 100 single-nucleotide substitutions in the bacteriophage f1, a filamentous single-strand DNA virus. We found that approximately one-fifth of all mutations are lethal. Viable ones reduced fitness by 11% on average and were accurately described by a log-normal distribution. More than 90% of synonymous substitutions were selectively neutral, while those affecting intergenic regions reduced fitness by 14% on average. Mutations leading to amino acid substitutions had an overall mean deleterious effect of 37%, which increased to 45% for those changing the amino acid polarity. Interestingly, mutations affecting early steps of the infection cycle tended to be more deleterious than those affecting late steps. Finally, we observed at least two beneficial mutations. Our results confirm that high mutational sensitivity is a general property of viruses with small genomes, including RNA and single-strand DNA viruses infecting animals, plants, and bacteria.

Abstract: In this paper we evaluate sources of background of the gamma p -> omega p reaction, with the omega detected through its pi(0)gamma decay channel, to compare with the experiment carried out at ELSA. We find background from gamma p -> pi(0)pi(0)p followed by decay of a pi(0) into two gamma, recombining one pi(0) and one gamma, and from the gamma p -> pi(0)eta p reaction with subsequent decay of the eta into two photons. This background accounts for the data at pi(0)gamma invariant masses beyond 700 MeV, but strength is missing at lower invariant masses which was attributed to photon misidentification events, which we simulate to get a good reproduction of the experimental background. Once this is done, we perform an event mixing simulation to reproduce the calculated background and we find that the method provides a good description of the background. A closer look reveals this is accidental. We show that the mixed event generated background in the region of the omega mass and beyond is completely tied to the events at low pi(0)gamma invariant masses where the d sigma/dM(pi 0 gamma) distribution is much larger. This has as a consequence that the mixed event method produces the same background at high invariant masses independently of the actual background in that region, as a consequence of which, the method is unsuited to give the background at energies around the peak of the omega and beyond.

Abstract: We report measurements of electron pair production in elementary p + p and d + p reactions at 1.25 GeV/mu with the HADES spectrometer. For the first time, the electron pairs were reconstructed for n + p reactions by detecting the proton spectator from the deuteron breakup. We find that the yield of electron pairs with invariant mass Me+e- > 0.15 GeV/c(2) is about an order of magnitude larger in n + p reactions as compared to p + p. A comparison to model calculations demonstrates that the production mechanism is not sufficiently described yet. The electron pair spectra measured in C + C reactions are compatible with a superposition of elementary n + p and p + p collisions, leaving little room for additional electron pair sources in such light collision systems.