| |
Records |
Links |
|
Author |
Di Gregorio, E.; Staelens, M.; Hosseinkhah, N.; Karimpoor, M.; Liburd, J.; Lim, L.; Shankar, K.; Tuszynski, J.A. |
|
| |
Title |
Raman Spectroscopy Reveals Photobiomodulation-Induced α-Helix to β-Sheet Transition in Tubulins: Potential Implications for Alzheimer's and Other Neurodegenerative Diseases |
Type |
Journal Article |
| |
Year |
2024 |
Publication |
Nanomaterials |
Abbreviated Journal |
Nanomaterials |
|
| |
Volume |
14 |
Issue |
13 |
Pages |
1093 - 21pp |
|
| |
Keywords |
proteins; protein dynamics; protein structure; non-invasive therapies; low-level laser therapy; spectroscopy; amide bands; amide I; spectral decomposition |
|
| |
Abstract |
In small clinical studies, the application of transcranial photobiomodulation (PBM), which typically delivers low-intensity near-infrared (NIR) to treat the brain, has led to some remarkable results in the treatment of dementia and several neurodegenerative diseases. However, despite the extensive literature detailing the mechanisms of action underlying PBM outcomes, the specific mechanisms affecting neurodegenerative diseases are not entirely clear. While large clinical trials are warranted to validate these findings, evidence of the mechanisms can explain and thus provide credible support for PBM as a potential treatment for these diseases. Tubulin and its polymerized state of microtubules have been known to play important roles in the pathology of Alzheimer's and other neurodegenerative diseases. Thus, we investigated the effects of PBM on these cellular structures in the quest for insights into the underlying therapeutic mechanisms. In this study, we employed a Raman spectroscopic analysis of the amide I band of polymerized samples of tubulin exposed to pulsed low-intensity NIR radiation (810 nm, 10 Hz, 22.5 J/cm2 dose). Peaks in the Raman fingerprint region (300-1900 cm-1)-in particular, in the amide I band (1600-1700 cm-1)-were used to quantify the percentage of protein secondary structures. Under this band, hidden signals of C=O stretching, belonging to different structures, are superimposed, producing a complex signal as a result. An accurate decomposition of the amide I band is therefore required for the reliable analysis of the conformation of proteins, which we achieved through a straightforward method employing a Voigt profile. This approach was validated through secondary structure analyses of unexposed control samples, for which comparisons with other values available in the literature could be conducted. Subsequently, using this validated method, we present novel findings of statistically significant alterations in the secondary structures of polymerized NIR-exposed tubulin, characterized by a notable decrease in alpha-helix content and a concurrent increase in beta-sheets compared to the control samples. This PBM-induced alpha-helix to beta-sheet transition connects to reduced microtubule stability and the introduction of dynamism to allow for the remodeling and, consequently, refreshing of microtubule structures. This newly discovered mechanism could have implications for reducing the risks associated with brain aging, including neurodegenerative diseases like Alzheimer's disease, through the introduction of an intervention following this transition. |
|
| |
Address |
[Di Gregorio, Elisabetta; Staelens, Michael; Tuszynski, Jack A.] Univ Alberta, Fac Sci, Dept Phys, Edmonton, AB T6G 2E1, Canada, Email: michael.staelens@ific.uv.es |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Mdpi |
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN  |
|
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
WOS:001269841000001 |
Approved |
no |
|
| |
Is ISI |
yes |
International Collaboration |
yes |
|
| |
Call Number |
IFIC @ pastor @ |
Serial |
6204 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Barrientos, D.; Bellato, M.; Bazzacco, D.; Bortolato, D.; Cocconi, P.; Gadea, A.; Gonzalez, V.; Gulmini, M.; Isocrate, R.; Mengoni, D.; Pullia, A.; Recchia, F.; Rosso, D.; Sanchis, E.; Toniolo, N.; Ur, C.A.; Valiente-Dobon, J.J. |
|
| |
Title |
Performance of the Fully Digital FPGA-Based Front-End Electronics for the GALILEO Array |
Type |
Journal Article |
| |
Year |
2015 |
Publication |
IEEE Transactions on Nuclear Science |
Abbreviated Journal |
IEEE Trans. Nucl. Sci. |
|
| |
Volume |
62 |
Issue |
6 |
Pages |
3134-3139 |
|
| |
Keywords |
FPGA; front-end electronics; gamma-ray spectroscopy; germanium detectors |
|
| |
Abstract |
In this work we present the architecture and results of a fully digital Front End Electronics (FEE) read out system developed for the GALILEO array. The FEE system, developed in collaboration with the Advanced Gamma Tracking Array (AGATA) collaboration, is composed of three main blocks: preamplifiers, digitizers and preprocessing electronics. The slow control system contains a custom Linux driver, a dynamic library and a server implementing network services. This work presents the first results of the digital FEE system coupled with a GALILEO germanium detector, which has demonstrated the capability to achieve an energy resolution of 1.53% at an energy of 1.33 MeV, similar to the one obtained with a conventional analog system. While keeping a good performance in terms of energy resolution, digital electronics will allow to instrument the full GALILEO array with a versatile system with high integration and low power consumption and costs. |
|
| |
Address |
[Barrientos, D.; Bortolato, D.; Cocconi, P.; Gulmini, M.; Rosso, D.; Toniolo, N.; Valiente-Dobon, J. J.] Ist Nazl Fis Nucl, Lab Nazl Legnaro, I-35020 Padua, Italy, Email: diego.barrientos@lnl.infn.it |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Ieee-Inst Electrical Electronics Engineers Inc |
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0018-9499 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
WOS:000372013500005 |
Approved |
no |
|
| |
Is ISI |
yes |
International Collaboration |
yes |
|
| |
Call Number |
IFIC @ pastor @ |
Serial |
2612 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Krzysiek, M. et al; Gadea, A.; Huyuk, T.; Barrientos, D. |
|
| |
Title |
Study of the soft dipole modes in Ce-140 via inelastic scattering of O-17 |
Type |
Journal Article |
| |
Year |
2014 |
Publication |
Physica Scripta |
Abbreviated Journal |
Phys. Scr. |
|
| |
Volume |
89 |
Issue |
5 |
Pages |
054016 - 6pp |
|
| |
Keywords |
pygmy dipole resonances; inelastic scattering; AGATA detector; gamma spectroscopy |
|
| |
Abstract |
The main aim of this study was a deeper understanding of the nuclear structure properties of the soft dipole modes in Ce-140, excited via inelastic scattering of weakly bound O-17 projectiles. An important aim was to investigate the 'splitting' of the PDR into two parts: a low-energy isoscalar component dominated by neutron-skin oscillations and a higher-energy component lying on the tail of the giant dipole resonance of a rather isovector character. This was already observed for this nucleus, investigated in (alpha, alpha') and (gamma,gamma') experiments. The experiment was performed at Laboratori Nazionali di Legnaro, Italy. Inelastic scattering of O-17 ion beam at 20 MeV A(-1) was used to excite the resonance modes in the Ce-140 target. Gamma-rays were registered by five triple clusters of AGATA-Demonstrator and nine large volume scintillators (LaBr3). The scattered O-17 ions were identified by two Delta E – E Si telescopes of the TRACE array mounted inside the scattering chamber. The telescopes consisted of two segmented Si-pad detectors, each of 60 pixels. Very preliminary data have shown a strong domination of the E1 transitions in the 'pygmy' region with a character more similar to the one obtained in alpha scattering experiment. |
|
| |
Address |
[Krzysiek, M.; Kmiecik, M.; Maj, A.; Bednarczyk, P.; Ciemala, M.; Fornal, B.; Grebosz, J.; Mazurek, K.; Meczynski, W.; Zieblinski, M.] Polish Acad Sci, H Niewodniczanski Inst Nucl Phys, Krakow, Poland, Email: mateusz.krzysiek@ifj.edu.pl |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Iop Publishing Ltd |
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0031-8949 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
WOS:000335785300016 |
Approved |
no |
|
| |
Is ISI |
yes |
International Collaboration |
yes |
|
| |
Call Number |
IFIC @ pastor @ |
Serial |
1790 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Pajtler, M.V. et al; Gadea, A. |
|
| |
Title |
Excited states of Y-90,Y-92,Y-94 populated in Zr-90+Pb-208 multinucleon transfer reaction |
Type |
Journal Article |
| |
Year |
2021 |
Publication |
Physica Scripta |
Abbreviated Journal |
Phys. Scr. |
|
| |
Volume |
96 |
Issue |
3 |
Pages |
035305 - 7pp |
|
| |
Keywords |
multinucleon transfer reactions; gamma spectroscopy; magnetic spectrometers; gamma-ray spectrometers |
|
| |
Abstract |
Multinucleon transfer reactions in Zr-90+Pb-208 have been studied via fragment-gamma coincidences, employing the PRISMA magnetic spectrometer coupled to the CLARA gamma-array. An analysis on Y isotopes has been carried out incorporating spectroscopic as well as reaction mechanism aspects. New gamma transitions have been observed in Y-94, confirming the findings of recent studies where nuclei were produced via fission of uranium, and a comparison with near-by Y-90,Y-92 isotopes populated in the same reaction has been discussed. Experimental cross sections have been extracted and compared with the GRAZING calculations, showing a fair agreement along the neutron pick-up side. The results confirm how multinucleon transfer reactions are a suitable mechanism for the study of neutron-rich nuclei. |
|
| |
Address |
[Pajtler, M. Varga] Univ Osijek, Dept Phys, Osijek, Croatia, Email: Suzana.Szilner@irb.hr; |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Iop Publishing Ltd |
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0031-8949 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
WOS:000611517400001 |
Approved |
no |
|
| |
Is ISI |
yes |
International Collaboration |
yes |
|
| |
Call Number |
IFIC @ pastor @ |
Serial |
4694 |
|
| Permanent link to this record |
| |
|
| |
|
Author |
Albaladejo, M.; Bibrzycki, L.; Dawid, S.M.; Fernandez-Ramirez, C.; Gonzalez-Solis, S.; Hiller Blin, A.N.; Jackura, A.W.; Mathieu, V.; Mikhasenko, M.; Make, V.I.; Passemar, E.; Pilloni, A.; Rodas, A.; Silva-Castro, J.A.; Smith, W.A.; Szczepaniak, A.P.; Winney, D. |
|
| |
Title |
Novel approaches in hadron spectroscopy |
Type |
Journal Article |
| |
Year |
2022 |
Publication |
Progress in Particle and Nuclear Physics |
Abbreviated Journal |
Prog. Part. Nucl. Phys. |
|
| |
Volume |
127 |
Issue |
|
Pages |
103981 - 75pp |
|
| |
Keywords |
Hadron spectroscopy; Exotic hadrons; Three-body scattering; Resonance production |
|
| |
Abstract |
The last two decades have witnessed the discovery of a myriad of new and unexpected hadrons. The future holds more surprises for us, thanks to new-generation experiments. Understanding the signals and determining the properties of the states requires a parallel theoretical effort. To make full use of available and forthcoming data, a careful amplitude modeling is required, together with a sound treatment of the statistical uncertainties, and a systematic survey of the model dependencies. We review the contributions made by the Joint Physics Analysis Center to the field of hadron spectroscopy. |
|
| |
Address |
[Albaladejo, Miguel; Blin, Astrid N. Hiller; Jackura, Andrew W.; Mokeev, Victor, I; Passemar, Emilie; Rodas, Arkaitz; Szczepaniak, Adam P.] Thomas Jefferson Natl Accelerator Facil, Theory Ctr & Phys Div, Newport News, VA 23606 USA, Email: alessandro.pilloni@unime.it |
|
| |
Corporate Author |
|
Thesis |
|
|
| |
Publisher |
Elsevier |
Place of Publication |
|
Editor |
|
|
| |
Language |
English |
Summary Language |
|
Original Title |
|
|
| |
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
| |
Series Volume |
|
Series Issue |
|
Edition |
|
|
| |
ISSN |
0146-6410 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
WOS:000883770300003 |
Approved |
no |
|
| |
Is ISI |
yes |
International Collaboration |
yes |
|
| |
Call Number |
IFIC @ pastor @ |
Serial |
5422 |
|
| Permanent link to this record |
