Abstract: Pauli blocking is carefully investigated for the processes of NN <-> N Delta and Delta -> N pi in heavy-ion collisions, aiming at a more precise prediction of the pi(-)/pi(+) ratio which is an important observable to constrain the high-density symmetry energy. We use the AMD + JAM approach, which combines the antisymmetrized molecular dynamics for the time evolution of nucleons and the Jet AA Microscopic transport model to treat processes for Delta resonances and pions. As is known in general transport-code simulations, it is difficult to treat Pauli blocking very precisely due to unphysical fluctuations and additional smearing of the phase-space distribution function, when Pauli blocking is treated in the standard method of JAM. We propose an improved method in AMD + JAM to use the Wigner function precisely calculated in AMD as the blocking probability. Different Pauli blocking methods are compared in heavy-ion collisions of neutron-rich nuclei, Sn-132+Sn-124, at 270 MeV/nucleon. With the more accurate method, we find that Pauli blocking is stronger, in particular for the neutron in the final state in NN -> N Delta and Delta -> N pi, compared to the case with a proton in the final state. Consequently, the pi(-)/pi(+) ratio becomes higher when the Pauli blocking is improved, the effect of which is found to be comparable to the sensitivity to the high-density symmetry energy.