Abstract: In this Letter we analyse, in the context of the general 2-Higgs Doublet Model, the structure of the Yukawa matrices, (Y) over tilde (q)(1,2), by assuming a four-zero texture ansatz for their definition. In this framework, we obtain compact expressions for (Y) over tilde (q)(1,2), which are reduced to the Cheng and Sher ansatz with the difference that they are obtained naturally as a direct consequence of the invariants of the fermion mass matrices. Furthermore, in order to avoid large flavour violating effects coming from charged Higgs exchange, we consider the main flavour constraints on the off-diagonal terms of Yukawa texture ((chi) over tilde (q)(j))(kl) (k not equal l). We perform a chi(2)-fit based on current experimental data on the quark masses and the Cabibbo-KobayashiMaskawa mixing matrix V-CKM. Hence, we obtain the allowed ranges for the parameters (Y) over tilde (q)(1,2) at 1 sigma for several values of tan beta. The results are in complete agreement with the bounds obtained taking into account constraints on Flavour Changing Neutral Currents reported in the literature.