Abstract: The first measurement of heavy-flavor production by the LHCb experiment in its fixed-target mode is presented. The production of J/psi and D-0 mesons is studied with beams of protons of different energies colliding with gaseous targets of helium and argon with nucleon-nucleon center-of-mass energies of root s(NN) = 86.6 and 110.4 GeV, respectively. The J/psi and D-0 production cross sections in pHe collisions in the rapidity range [2, 4.6] are found to be sigma(J/psi) = 652 +/- 33(stat) +/- 42(syst) nb/nucleon and sigma(D0) = 80.8 +/- 2.4(syst) +/- 6.3(syst) μb/nucleon, where the first uncertainty is statistical and the second is systematic. No evidence for a substantial intrinsic charm content of the nucleon is observed in the large Bjorken-x region.