Abstract: We describe the main features of a new and updated version of the program PArthENoPE, which computes the abundances of light elements produced during Big Bang Nucleosynthesis. As the previous first release in 2008, the new one, PArthENoPE2.0, is publicly available and distributed from the code site, http://parthenope.na.infn.it . Apart from minor changes, which will be also detailed, the main improvements are as follows. The powerful, but not freely accessible, NAG routines have been substituted by ODEPACK libraries, without any significant loss in precision. Moreover, we have developed a Graphical User Interface (GUI) which allows a friendly use of the code and a simpler implementation of running for grids of input parameters. New Version program summary Program Title: PArthENoPE2.0 Program Files doi : http://dx.doi.org/10.17632/wvgr7d8yt9.1 Licensing provisions: GPLv3 Programming language: Fortran 77 and Python Supplementary material: User Manual available on the web page http://parthenope.na.infn.it Journal reference of previous version: Comput. Phys. Commun. 178 (2008) 956 971 Does the new version supersede the previous version?: Yes Reasons for the new version: Make the code more versatile and user friendly Summary of revisions: (1) Publicly available libraries (2) GUI for configuration Nature of problem: Computation of yields of light elements synthesized in the primordial universe Solution method: Livermore Solver for Ordinary Differential Equations (LSODE) for stiff and nonstiff systems