Abstract: We report measurements of charm-mixing parameters based on the decay-time-dependent ratio of D-0 -> K+pi(-) to D-0 -> K-pi(+) rates. The analysis uses a data sample of proton-proton collisions corresponding to an integrated luminosity of 5.0 fb(-1) recorded by the LHCb experiment from 2011 through 2016. Assuming charge-parity (CP) symmetry, the mixing parameters are determined to be x'(2) = (3.9 +/- 2.7) x 10(-5), y' = (5.28 +/- 0.52) x 10(-3), and R-D = (3.454 +/- 0.031) x 10(-3). Without this assumption, the measurement is performed separately for D-0 and (D) over bar (0) mesons, yielding a direct CP-violating asymmetry A(D) = (-0.1 +/- 9.1) x 10(-3), and magnitude of the ratio of mixing parameters 1.00 < vertical bar q/p vertical bar < 1.35 at the 68.3% confidence level. All results include statistical and systematic uncertainties and improve significantly upon previous single-measurement determinations. No evidence for CP violation in charm mixing is observed.