Abstract: We perform the first measurement on the D-0 – (D) over bar (0) mixing parameters using a time-dependent amplitude analysis of the decay D-0 -> pi(+)pi(-)pi(0). The data were recorded with the BABAR detector at center-of-mass energies at and near the Upsilon(4S) resonance, and correspond to an integrated luminosity of approximately 468.1 fb(-1). The neutral D meson candidates are selected from D*(2010)(+) -> D-0 pi(+)(s) decays where the flavor at the production is identified by the charge of the low-momentum pion, pi(+)(s). The measured mixing parameters are x = (1.5 +/- 1.2 +/- 0.6)% and y = (0.2 +/- 0.9 +/- 0.5)%, where the quoted uncertainties are statistical and systematic, respectively.