Abstract: We present a measurement of D-0-(D) over bar (0) mixing and CP violation using the ratio of lifetimes simultaneously extracted from a sample of D-0 mesons produced through the flavor-tagged process D*(+) -> D-0 pi(+), where D-0 decays to K+pi(+/-), K-K+, or pi(-)pi(+), along with the untagged decays D-0 -> K+pi(+/-) and D-0 -> K-K+. The lifetimes of the CP-even, Cabibbo-suppressed modes K-K+ and pi(-)pi(+) are compared to that of the CP-mixed mode K+pi(+/-) in order to measure y(CP) and Delta Y. We obtain y(CP) = [0.72 +/- 0.18(stat) +/- 0.12(syst)]% and Delta Y = [0.09 +/- 0.26(stat) +/- 0.06(syst)]%, where Delta Y constrains possible CP violation. The y(CP) result excludes the null mixing hypothesis at 3.3 sigma significance. This analysis is based on an integrated luminosity of 468 fb(-1) collected with the BABAR detector at the PEP-II asymmetric-energy e(+)e(-) collider.